Analysis program of magnetic particles movement has been developed for the purpose of the prediction of path of magnetic particles in a flow cell of an immunoassay detector. Magnetic particles flow into the flow cell composed of a channel, electrodes, and a magnet. These particles are captured by the magnet below the electrode. It is very important to calculate and predict the path of particles for a design of high-precision detector. Forces such as drag force, pressure gradient force, gravity force, and magnetic force are accounted for the magnetic particles. Comparison between computational and experimental particles distribution on the electrode were made, and predicted adsorbed particles distribution is good agreement with experimental one. Proposed methods and developed program are very effective for the prediction of magnetic particles movement.