Biocompatible honeycomb-patterned polymer films (HF) as a precision micro-porous arrayed scaffold were tested to proliferate periodontal ligament cells for the periodontal regeneration therapy. HFs were prepared from poly(ε-caprolactone) (PCL) and amphiphilic, with 5 or 10 μm diameter of precise pores casted by water droplet array method. Periodontal ligament (PDL) cells from 6 extracted human molars were cultured on HFs up to 28 days. After 24h culture, PDL cells attached to pillars in the honeycomb structure of HF, and shaped like pseudopodium form. On the 10 μm pored HFs, cells entered into the film and migrated to the structural lumen. At 28-day culture, PDL cells on HFs formed the multilayered cell sheet like-structure with the penetration of cells. HF is applicable to the periodontal therapy as a cell-interactive scaffold for the periodontal tissue regeneration.
