J0202-1-3 Variation of endothelial cells strain energy density in response to mechanical stimuli

Abstract

The present study demonstrated that exposure of cultured bovine aortic endothelial cells (BAEC) to fluid flow resulted in nitric oxide (NO) production and up-regulation of intracellular calcium ion concentration ([Ca^<2+>]_i), monitored by fluorescent dyes of DAF2-DA and Rhod2-DA, respectively using a confocal laser scanning microscope. BAEC were exposed to the pulsatile flow of culture media for 5 minutes. Distribution of NO concentration associated with Ca^<2+> concentration distribution were observed after 5 minutes exposure of the level of 2 Pa shear stress. Results implies that most of the amount of NO was produced by means of Ca^<2+> dependent activation of endothelial nitric oxide syntase (eNOS). Expression of endothelial and inducible NO synthase were evaluated at the protein level. The expression of eNOS in the shear loaded BAEC was detected by the methods of immuno staining (Western Blot) whereas that of iNOS was not confirmed.