This paper describes observations of thrombus formation process on orifice flows by laser sheet and velocity measurements by PIV method in the same circuit. In the observation of thrombus formation process, the brightness of CCD image corresponds to protein or thrombus concentration. In this paper, thrombus formation rate was estimated by the gradient of brightness history near the wall, hi the PIV measurements, shear stress distribution on each orifice was obtained. Comparing the thrombus rate with the shear stress distribution on each orifice, it was found that high shear stress region affects the thrombus rate in the orifice flow.