J0202-2-1 Development of a Plate Type Microstructures Bioreactor for High Density Cell Culture

Abstract

An engineered tissue performing in-vivo physiological metabolic rate should keep cellular density around 10^8〜10^<10> cells/mL, which is far higher than that of normal dish culture. This study proposes a perfusion culture plate with designed microstructure allowing HepG2 cells of 10^7〜10^8 cells/mL to adhere on the plate. The total oxygen metabolic rate of the bioreactor was calculated using the cellular adhesion ratio to fluid shear flow stress and the single cellular oxygen metabolic rate. The measured oxygen metabolic rate is lower than the calculated values, although the trends of oxygen metabolic rate to perfusion flow rate for both measured and calculated are similar. It is suspected that the cellular high oxygen consumption rate in the still medium in the bioreactor might lead to lethal hypoxic condition in a short time before starting the perfusion.