Abstract
We developed a DNA separation chip utilizing micrometer-sized fence structures fabricated in a micro channel. The separation was achieved based on the principle of size exclusion chromatography (SEC). The smaller DNA molecules take longer time to pass through the molecular sieve of fences. In this study, we integrated a nano-slit structure for the pre-concentration of DNA samples in the same micro-channel. Since the pre-concentration was conducted just before the separation, we improved the resolution of DNA separation and succeeded in separating DNA molecules of 2 kbp and 10 kbp.
