Fatigue tests have been conducted to investigate the influence of irreversible hydrogen and low temperature annealing on the fatigue strength for a cold drawn eutectoid steel specimen. For both as-received and annealed samples, crack initiated at internal inclusion. In the case of as-received samples, irreversible hydrogen had no influence on the fatigue strength. However, for the annealed samples, irreversible hydrogen caused a decrease in fatigue strength. These results indicate that a decrease in fatigue strength by absorbing irreversible hydrogen appears remarkably by low temperature annealing.