In order to examine a possibility of hydrogen as a tracer for evaluating fatigue damage accumulation, the fatigue damaged carbon steels have been charged with hydrogen by cathodic electrolysis. The hydrogen-charged steels have been subjected to the TDS analysis after exposing them to atmosphere for 5 mm. In this study, the specimen of which the surfaces except the fatigue damaged one were coated with epoxy resin was subjected to the hydrogen-charging and the duration of exposure time was prolonged to be 30 mm for improving the detection sensitivity. Experimental results revealed that the temperature of hydrogen evolution peak, which appeared at around 85-90℃ during the TDS analysis irrespective of the degree of fatigue damage, increased by 15℃ when compared with the temperature measured by the conventional method. In addition, the differences between the amount of desorbed hydrogen, C_H, of the virgin steel and those of the fatigue damaged specimens were slightly pronounced by the above modification.