In order to investigate the relationship between hydrogen induced hardening of austenitic stainless steel and the hydrogen content, the spherical micro-indentation tests and thermal desorption analyses have been conducted on hydrogen charged austenitic stainless steel JIS SUS316L. Hydrogen has been charged by using cathodic method. In order to change the hydrogen content, current density and charging time are changed from 0.01 mA/mm^2 to 1.0 mA/mm^2, from 0 h to 48 h respectively. The obtained results show the hardness increase along with charging current density and charging time. Hydrogen content also depends on the charging current density and charging time. These relationships result in the linear correlation between hydrogen induced hardening of austenitic stainless steel and the hydrogen content. Hydrogen enhanced hardening might be due to dislocation-pinning effect of hydrogen. Thus there is a possibility to apply micro-indentation tests for measurements of hydrogen content.