In this study, the effect of low temperature nitriding on the 4-points bending fatigue properties of Ti-6Al-4V alloy were examined. The fatigue limit of the nitrided specimens tended to increase as the nitriding temperature decreased. Especially, the specimens nitrided at 550 ℃ showed higher fatigue limit than that of the un-nitrided specimen, whereas the conventional nitriding at 850 ℃ reduced the fatigue limit of Ti-6Al-4V alloy. This was because a thick nitrogen compound layer was formed and grain-coarsening occurred. Moreover, the fatigue crack propagation of the nitrided Ti-6Al-4V alloy was observed using the developed system.