皮質骨有機・無機ラマンシフトの負荷応答と加齢の影響

抄録

In recent years, bone diseases such as osteoporosis, which tend to cause fractures due to a decrease in bone strength accompanying aging, are becoming a problem. Evaluating bone microscopic mechanical properties and evaluating bone strength is an important research leading to the treatment of bone diseases. Using the component analysis method called Raman spectroscopy, the change in Raman shift was measured while applying load to bone tissue, and the relationship between stress and Raman shift was investigated. In addition, the Raman shifts were measured by statistical process based on the Raman images. The specimens with the sizes of 10 × 2 × 0.5 mm were cut out from bovine femoral diaphysis (Age: 1 m.o., 23 m.o. and 7 y.o.), where the longer edges of specimens are aligned to the parallel to the femoral axis. Strain gauges were attached to bovine cortical bone specimens, and Raman spectrum was measured while applying tensile stress. The Raman shifts were calculated by averaging obtained peak shifts at all measurements point. As the results, a negative correlation was found between tensile load and Raman shift in ν₂PO₃^4－ , ν₄PO₃^4－ and ν₁PO₃^4－. In addition, at 23 months of age, there was a tendency of the increase of changes in Raman shifts.