がん細胞スフェロイドが浸潤する際の細胞外マトリックスの応力場計測

抄録

When tumor cells metastasize, invasion of cells plays a major role. The tumor cells initiate invasion by generating force and changing the physical structure of the extracellular matrix (ECM) surrounding the tumor. However, the detail process of changing the structure of the ECM has not been elucidated. At this time, measuring the mechanics field generated in the ECM is an important way to understand behavior of tumor cells in 3D. Therefore, in this study, spheroids, which are the source of the invasion as a tumor cells, were embedded in a collagen gel, and the deformations of the collagen gel surrounding the spheroids were measured. Using the DVC method, the matrix deformations around spheroids were measured by tracking the 3D positions of fluorescent beads embedded in the collagen gel. To photograph the initial state of the ECM, we added tritonX-100 to the spheroid at the end of the experiment and photographed the state of no stress from the spheroid. In addition, we calculated the divergence of the deformations field and discussed on density change of ECM. As a result, it was found that spheroids systematically generate the deformation field and change the density of ECM. It was suggested that invasion occurs in the low density part.