Abstract
This paper proposes a mechanical liposome or cell contact system using a parylene filter and gentle downward flow for the function analysis of membrane proteins. Membrane proteins play an important role in several biological functions including signal transduction, energy production and cellular communication. For the analysis of membrane proteins, the methodology of liposome and cell trap or contact is highly demanded. In this paper, we developed the device that can trap a single liposome or multiple cells on parylene filter. By using this device, the fluorescence dye transports mediated by membrane proteins were successfully observed under confocal laser scanning microscopy. We believe that this device can accelerate the study of membrane proteins.
