Abstract
In this study, we have developed a microfluidic device integrated with a microfluidic probe to clarify a differentiation mechanism of pluripotent stem cells. It is difficult to evaluate efficacy of chemical reagents for the differentiation using a conventional method because cells cultured in culture dishes are exposed to the reagents homogenously. Here, we propose a novel culture method which enables exposing cells to different chemical condition by location. The device was evaluated by a computer simulation using FEM, and a flow control experiment. A control of chemical stimulation from single cell level to tissue level in a microspace could be possible by regulating flow ratio and channel size of the microfluidic probe.
