5PM3-PMN-029 High-throughput antibody screening device toward embryo assay

Abstract

In embryology, researchers conduct a screening of a monoclonal antibody for identifying specific protein, but the process takes long time by conventional techniques. In this study, we propose a microfluidic device which is capable to expose multi-embryos to different reagents each at a time. The device has 16 inlets and reaction chambers, and their reagents can be flowed to one outlet by a syringe pump. An immunostaining process was carried out to evaluate a function of the device. As a result, it was confirmed that the reaction time could be shortened, and volume of reagents could be reduced in comparison with conventional methods.