抄録
In this study, we have developed a microfluidic device to control chemical stimulation to a cell population at a single cell scale for cell-based assay. The device consists of a cell culture chamber and microfluidic probe (MFP) channels which are possible to control pinpoint chemical stimulation in a microspace. The device has been evaluated by a computer stimulation using Finite Element Method (FEM) and a flow control experiment. A control of chemical stimulation from single cell scale to a cell population scale in the chamber can be possible by regulating flow ratio and channel size of the microfluidic probe. We conclude that the device can be a powerful tool for biological applications such as the differentiation control of pluripotent stem cells and other cells signaling studies.
