Abstract
We developed the microwell device for measurement of membrane transport of single adherent cells. As the cells in a population (e.g., tumor) are inevitably heterogeneous, a technique to measure the transport activities at a single-cell level is needed. When adherent cells were cultured on the microwells with 〜10 μm diameter, they spread over the opening to form the closed picoliter space. Thus, molecules exported from cells accumulate in such a space and be detected by fluorescent imaging. In this report, we studied the microwell structure to improve the efficiency of closed-space formation and the yield of transport measurement.
