Development of a portable device for biological nanopore recordings with a liquid-capturing system

Abstract

We developed a portable device for biological nanopore recordings with a liquid-capturing system. Recently, biological naopore sensors increasingly attract attentions, but their stability is still an important issue for their mobile applications. Hence, we combined the cup-like reservoir with a glass capillary–based lipid bilayer device. The reservoir, a cylindrical well with three claws, covers the capillary tip and allows to catch the solution by the surface tension. We formed a lipid bilayer on the tip of the capillary by a conventional painting method and reconstituted nanopore into the lipid bilayer. We succeeded in monitoring an ionic current by α-hemolysin incorporation and DNA translocations through the nanopore.