マイクロ・ナノ工学シンポジウム
Online ISSN : 2432-9495
セッションID: PN-136
会議情報

溶液把持機構を有するポータブル生体ナノポア電気計測デバイスの開発
*伊沢 友佑大崎 寿久神谷 厚輝藤井 聡志三澤 宣雄三木 則尚竹内 昌治
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会議録・要旨集 認証あり

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We developed a portable device for biological nanopore recordings with a liquid-capturing system. Recently, biological naopore sensors increasingly attract attentions, but their stability is still an important issue for their mobile applications. Hence, we combined the cup-like reservoir with a glass capillary–based lipid bilayer device. The reservoir, a cylindrical well with three claws, covers the capillary tip and allows to catch the solution by the surface tension. We formed a lipid bilayer on the tip of the capillary by a conventional painting method and reconstituted nanopore into the lipid bilayer. We succeeded in monitoring an ionic current by α-hemolysin incorporation and DNA translocations through the nanopore.
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© 2017 一般社団法人 日本機械学会
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