Automated Single-Cell Collection Technology Based on Photocuring of Gelatin by Multipoint Light Irradiation

Abstract

This study aims to develop an automated single-cell collection technology based on photocuring of gelatin methacrylate (Gel-MA) by multipoint light irradiation. Target single cells were observed through an inverted microscope, and irradiation pattern was automatically generated through image processing. A processed image was used to cure Gel-MA around the target cells. The viability of cells at various light irradiation time was measured. In future, residual Gel-MA will be removed by the biodegradable property of Gel-MA.