This study aims to make clear the vacancy controllability of inverse opal structure fabricated by patterned and repeated dip-coating The process consists of two steps of coating The first one is fabrication of sacrificial particles assembly, silica in this case The second one is fabrication of nano-particles for shell-structure around the sacrificial particles After these processes, silica particles were selectively dissolved to fabricate inverse opal or porous structures It was made clear that vacancy of inverse opal can be controlled by changing particle diameter We also demonstrate the fabrication of patterned inverse opal structure by using hydrophilic/hydrophobic patterned substrate