Abstract
Near infra-red (NIR) diffuse optical tomography (DOT) has gained much attention for recent years because it is noninvasive and deeply penetrating to biologic tissue. Here we report our reconstruction results of an in vitro chicken leg and an in vivo exercising human forearm using a multi-channel time-resolved NIR system and a two-dimensional image reconstruction algorithm based on a modified generalized pulse spectrum technique for simultaneous reconstruction of the absorption and scattering coefficients. The images revealed the anatomies of a chicken leg and a human forearm, and the bones were clearly distinguished from the muscle. The absorption images showed the blood volume changes during the forearm exercise, proving that the system and the algorithm could potentially be used for imaging not only the anatomy but also the hemodynamics in living tissues.
