Abstract
Laser tweezers are suitable for manipulation of a single microscopic object. Single cell manipulation is important for biological research works. We proposed indirect manipulation of cell with the laser trapped microtools to prevent heat damage by direct lighting of laser at a cell. In this report, we succeeded in fixation and pinpoint injection of microtools in a microchip. ITO microelectrodes are patterned on the surface of the microchannel by photolithography. The microtools are fixed on the electrode with the gelatin layer beforehand. To release the fixed tools in water, these electrodes work as micro heaters to melt the gelatin layer. Then, the released tools are caught in the microchannel by dielectrophoretic force near the electrodes. These electrodes are used to generate electric field gradient near the tools. Effectiveness of the proposed method is demonstrated by the experiment.
