Abstract
We evaluated the metabolic activity of HEK 293 cells using a pH/CO_2 sensor system using two ion-sensitive field effect transistors (ISFETs). The system could be utilized to evaluate the production rate of total carbonate and free lactic acid. The quantitative analysis of metabolic switching by the change of glucose concentrations indicated that a decrease of glucose concentration increased total carbonate that represents the cellular respiration activity and decreased free lactic acid that represents glycolysis activity. We analyzed a switching of metabolic pathways from glycolysis to respiration with a decrease of glucose quantitatively in HEK 293 cells for the first time.
