Abstract
In this paper, we realize the local cell culture and single cell harvesting by using the semi-closed microchip, which is the microchip we proposed to realize the probe type manipulation in the microchip. To use the semi-closed microchip for cell culture and cell analysis, the exchange of the solution in the bath was demonstrated. It was also demonstrated that the cell fixation with the thermo sensitive gel in the semi-closed microchip by fabricating the ITO electrodes as heaters in the bath. The semi-closed microchip can be used for cell culture, cell analysis and cell harvesting as the biological applications for single cell analysis.
