Abstract
Trapping the cell at the target position with minimal stress and reconstructing the same 3D cell environment as in vivo are the key technologies for the progress of the biological studies since they are essential technologies to obtain the reliable experimental data both in elucidating the pathological mechanisms and in creating the medicine. This paper proposed a new micro-fluidic cell culturing system which can trap the cell at the center of the microscopic field in the micro-fluid channel by constructing the suitable flow rate patterns. In the proposed system, the position of the target cell is traced by using the image processing technologies and the target cell is trapped by switching the rate pattern of the culturing solution injected from the branch channels according to the observed current cell positions. The cell trapping abilities of the developed system were examined through some experiments.
