Abstract
This paper presents spatially arranged microelectrodes to allow electrophysiological recordings inside 3D neuronal cultures. Out-of-plane microelectrode probes standing on a substrate have gradation in height. Wire-bonding-based probe technology makes it possible to provide the flexible probes. We would understand the dependent alteration of cellular activities caused by the construction process of 3D artificial neuronal networks, because the developed probes can directly and spatially record cellular activities inside 3D cell cultures. This paper demonstrated electrophysiological activities inside 3D neuronal cultures could be successfully recorded using the embedded probes. Given the results described here, further electrophysiological characterizations of 3D neuronal cultures using our spatially arranged microelectrodes are underway toward bioengineering applications such as: disease models for the study of regeneration and repair of damaged nervous systems, specifically Alzheimer's disease.
