Abstract
A microbe contains various enzymes enabling multi-step biochemical reaction. Extremely wide varieties of bacteria have been discovered or genetically modified to enhance the process efficiency and to develop new biochemical reactions. To investigate the performances of microbes, it is necessary to not only quantify the number of microbes that are involved in the reaction but also make their reaction conditions consistent. Therefore precise control and immobilization of microbes is crucial. In this paper we propose bacteria handling and immobilization in micro scale holes by dielectrophoresis. This way can control precise amount of bacteria, identify the number of the bacteria and analyze their reaction accurately. We succeeded in handling and immobilizing bacteria in micro scale holes. The number of immobilized bacteria could be controlled and only alive bacteria were successfully collected.
