1A1-V04 Development of Implantable Drug Delivery Systems Using PDMS Pneumatic Balloons(MEMS and Nano-Technology)

Abstract

Recently, it is considered that in vivo transfection is an important technique used in biological research and drug therapy development. In this paper, we report a novel implantable microsystem for delivery of nucleic acid drugs to mice kidney. The microsystem consists of pneumatically actuated balloons, renal case, and pressure-supplying port. Renal pressure-mediated transfection of plasmid DNA was successfully performed by using the microsystem implanted into mice. The highest expression of transfected plasmid DNA was obtained when the microsystem was activated at 60 kPa of pneumatic pressure for 10 s. The developed microsystem is valuable for in vivo gene functional analysis, diseased animal development, and preclinical studies of gene therapies.