1A2-Q11 Automatic Presumption of the Three-Dimensional Position and Posture of a Bacteria using the Bright Field Microscopic Images from Two Directions(Bio-Mimetics and Bio-Mechatronics(2))

Abstract

Two synchronized movies where bacteria are swimming near a surface are analyzed to obtain three dimensional position and posture of a bacterium. Our microscopy has two objective lenses that are orthogonal and fixed during the observation. Since the blurred contours of the bacteria, due to the fixed objective lenses, and large amount of images in the movie make the measurement cumbersome, we have developed a program that automatically presumes the position and posture of the bacterium. From the images obtained by bright field microscopy, the position and posture of a cell of elongated shape are obtained by approximation with an ellipse. Position of cells, which have the size of a few micrometers, is presumed to an accuracy of 0.7 micrometers, while posture is difficult to presume when cells look like circle because of the blurring.