Abstract
A patterning technique of cells is required for studying cell communication between multiple cells. The objective of this research is to develop an array of micromanipulators which can pattern cells with high precision and in parallel. A cell transportation device is developed to carry cells to nozzles and decrease the suction pressure. We investigated the optimal condition of cell manipulation with a glass pipette. The diameter of the pipette is about the same diameter as a nozzle of the micro-manipulator array to mimic the experimental conditions. Mechanical deformation of captured cells was observed through the microscope. 15 μm was aspirated into the pipette in one second. Probability of cell release from the pipette increased as trapped time of cell decreased.
