Abstract
Construction of large tissues from cell aggregates is one of the promising methods for tissue engineering applications. Compared to spherical cell aggregates, non-spherical cell aggregates are more suitable as building blocks to be assembled into complex larger structures. In this study, we prepared Ca-alginate hydrogel plates with microchamber structures via micromolding using a mixed solution of sodium alginate (NaA) and propylene glycol alginate (PGA) to form toroidal cell aggregates composed of multiple cell types. In addition, the prepared toroidal cell aggregates were used to construct capillary-like tissue structures by stacking through thin wires.
