Abstract
We developed a novel method called HCMP, High-performance Cell Membrane Perforation as a physicochemical transfection. In HCMP, pinpoint oxidative cell membrane degeneration which is induced by light-irradiated photosensitizer enables non-disruptive cell manipulation through submicrometer size cell membrane perforation. Any materials which can pass through the perforated area are transfectable. Conventional physicochemical transfection methods are relative safe but low efficiency than biological methods, but HCMP resulted in almost 100% survival as well as the successful transfection. The needle array-like cell membrane perforator is made by nano-imprinting with low-cost. Moreover, the method is simple, so we could automate the process by the robotics technology for the low-cost easy operation.
