Abstract
This study discusses interfacial analysis of cell sheet surface for evaluating the wetting characteristic of cell sheet quantitatively and noninvasively as much as possible. After squeezing culture media on the cell sheet by air-jet, the squeezed width of culture media is related to surface wettability of cell sheet by theoretical analysis. The squeezed width is correlated with the gene expression levels of mucin family, which is main component of mucosal surface.