Abstract
A patterning technique of cells is required for studying cell communication between multiple cells. The objective of this research is to develop a versatile cell manipulation station enabling pattern of single cells with high precision and in super parallel format. In this paper we developed a cell feeder to collect cells on microprobes and decrease manipulation force depending on the suction pressure. Electrodes of the cell feeder were etched from ITO substrates by MEMS fabrication processes. Application of alternate voltage of 5, 10, 15, 20_<Vpp> collected cells by dielectrophoresis. Cells were collected between the electrodes at 5 MHz by positive dielectrophoresis. For efficient cell capture, we combined an array of microprobes and a cell feeder and manipulated cells.
