Abstract
We developed selective injection of a single particle sensor using optically-regulated cell adhesion and injection for intracellular temperature measurement. Fluorescence sensor encapsulated in liposome is fabricated by spontaneous transfer method. The liposome is consists of neural lipids and spiropyran (SP). SP is a photochromic material and used for optical surface potential control of the liposome. The liposome adheres to the cell membrane by coulomb's force under UV illumination. The sensor can contain various fluorescence indicators such as Rhodmaine B as temperature sensor. The liposome encapsulating the sensor is contacted to the cell membrane using optical tweezers. We evaluated encapsulation efficiency of the sensor into the liposome and adhesion efficiency to cell membrane. We demonstrated the optically-controlled adhesion of the sensor to MDCK cell and observed the injected sensor by laser confocal fluorescence method. As results, we succeeded in injection of particle sensor into the cell cytoplasm and nucleus.
