Abstract
This study demonstrates to assemble label-free massive cells three-dimensionally in parallel and bridge between micropillars without any scaffold nor specific patterning. We already proposed the label-free magnetic manipulation based on the magneto-Archimedes effect and demonstrated spheroid array formation in a microfluidic chip. In the present study, we extended the manipulation method from two-dimensional (2D) to three dimensional (3D). By arranging Neodymium (Nd) magnets to generate a magnetic force not only on a horizontal direction but also on a vertical direction, cells were aggregated and levitated between micropillars. After incubation of several hours, the cells connected each other and bridged between micropillars.
