3A1-I02 Derivation of mechanical parameter of cell sheet by uniaxial tensile test(Bio Manipulation)

Abstract

In this study, we measured mechanical properties of cell sheet by using uniaxial tensile test system which we developed. We applied kelvin model (viscoelastic model) which applied single cells for deriving mechanical properties such as elastic modulus and viscous modulus. Mechanical properties of Fibroblast (NIH-3T3) cell sheet and myoblast (C2C12) cell sheet were derived by fitting equation which related kelvin model to measured stress-strain curves of cell sheet. Furthermore, for confirming drag response on cell sheet mechanical properties. By deriving mechanical properties of cell sheet, we confirmed mechanical properties of cell sheet which are different from that of single cells.