Abstract
This paper reports the development of a palm-size device called "PASMA (Pressure Actuated Shapable Microwell Array)" for formation, evaluation and collection of embryonic bodies (EBs) of stem cells. By incorporating a transparent heat film, water evaporation chamber and CO_2 generation reagents, i-PASMA realized miniaturization of the whole process of EB experiments in one chip without a conventional big incubator. By putting this system under the microscope, EB fabrication process from human induced pluripotent stem cells (hiPS cells) were successfully observed. The massively parallel analysis system "i-PASMA" should accelerate the regenerative medicine as a total EB research platform.
