1A1-D01 Development of Spheroid Morphological Evaluation System for Cells Construct in Regenerative Medicine

Abstract

In the field of regenerative medicine, 3-D tissue engineering, which intends to regenerate lost tissues and organs, is mainly focused. We have already established a technique to make a 3-D construct only with cells. Cells construct is made by spheroid-culturing the cells and neatly arrange the spheroids. Namely, quality of spheroids is important. If there is a difference in the number of cells when dispensing the cells turbid liquid, spheroids in different sizes are generated. Therefore, this study was aimed to develop the spheroid morphological evaluation system. We used mesenchymal stem cell of rabbit. Upon reaching the required number of cells, we were dispensed into the special multiwell plate for forming spheroids. We analyzed the quantitative evaluation about spheroids using the developed system. In result, it was found that there is a range of cell number to form spheroids with high degree of circularity.