Abstract
Platelet transfusions are expected to be short in the future due to the donor decrease and its 4-days short storage life. Therefore it is necessary to show the process of the production of platelets from megakaryocyte (MKs) in order to produce artificial platelets ex vivo in high rate. Therefore, having focused on the effect of shear stress on MKs, we proposed on-chip monitoring system of the MKs in shear flow environment. On-chip monitoring system consists of microfluidic chip, flow control part and observation part. It can apply controlled shear flow to MKs and monitor MKs because of its transparency. In this research, we fabricated microfluidic chip by using MEMS technique and demonstrated on-chip monitoring of the megakaryocyte. At last. we confirmed that the platelets collected from our chip had the characteristics receptor by using FACS analysis.
