The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)
Online ISSN : 2424-3124
2015
Session ID : 1P1-N03
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1P1-N03 On-chip fabrication of microstructures using the biodegradable material
Ryota SAKAKIBARAMasaru TakeuchiAkihiko ICHIKAWAKenichi OharaMasahiro NakajimaYasuhisa NakajimaToshio FUKUDA
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Abstract
In this paper, we conducted the fabrication of cell embedded structures using biodegradable photocrosslinkable material gelatin methacrylate (GelMA). The mouse fibroblast NIH3T3 cells were mixed in uncured GelMA solution. The cell embedded microcstructures were fabricated by exposing ultra violet (UV) light under a microscope. The cell culture was conducted to check their growth inside the microstructures. The cell viability was checked using live/dead cell staining. The observation of cell structures under a confocal microscope was conducted to check the three dimensional shape of the structure. The experimental results validate that the cells can grow inside the GelMA structures and the fabricated structures can be used to construct in vitro 3D cell structures for tissue engineering applications.
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© 2015 The Japan Society of Mechanical Engineers
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