Abstract
Mechanical interaction between cell and scaffold has close relations with various function of the cell. By analyzing the force exerted by cell which adhering to scaffold, it's expected that some new mechanism of the cell is revealed. As a method for measurement of interaction between cell and scaffold, Traction Force Microscopy (TFM) is generally used. In this method, fluorescence beads as marker is randomly mixed in hydrogel scaffold, and it's able to calculate the cell traction force from deformation of the scaffold calculated from beads' displacement information. In conventional method, confocal microscopy is often used for the measurement, and measurement time is not taken into consideration. In this research, by arranging beads into two layer of lattice shape in hydrogel, we aim to improve measurement speed.
