Abstract
In this paper, to improve the spatiotemporal resolution and to reduce the invasiveness, we genetically introduce Optically-Manipulated molecular switch, channelrhodopsin (ChR), into sarcoplasmic reticulum (SR) of C2C12 myoblast, mouse muscle cell lines. A myotube inserted with SR-targeted ChR can respond rapidlier than a myotube inserted with non-targeted ChR for 10-20 ms. A myotube inserted with SR-targeted ChR can contraction only by intracellular ion transportation. We can control intracellular Ca^<2+> ion of the myotube. This technique could facilitate the studies on the myogenesis, the stem cell therapy for muscular disorder, and the bioengineering using the muscle-powered actuator.
