Abstract
Single cell analysis has attracted much attention for revealing detailed and localized biological information. A local environmental control technique is desired when analyzing the detailed and localized properties of single cells. In this paper, we propose a local environmental stimulation system with micro dual-pipettes to stimulate the local reagent concentration dynamically, freely and automatically. When the local environment is controlled with chemical stimuli, the chemical solution is diffused and affects other objects. The local environment stimulation system with micro dual-pipettes can solve this problem by using spout pipette and suction pipette. For the evaluation of developed system, we used fluorescent substance and confirm controllability of local concentration quantitatively. Furthermore, we confirmed usefulness of this system by measuring and controlling the rotational speed of flagellar motor, and by peeling off certain area of adhered cells, as an application of the system.
