1P1-O10 Evaluation for C. elegans survival ratio after micro-injection

Abstract

We evaluated a survival rate of micro-injection into Caenorhabditis elegans, (C. elegans) after 24 hours with a fluorescent micro-gel bead. The fluorescent micro-gel beads were fabricated by a salting-out method. One of the micro-gel bead was picked up at the micropipette injection tool by suction forces. The microbead was injected into C. elegans by a microinjector using a piezo actuator based on micromanipulation. After microinjection of the fluorescent microbead, the microbead was confirmed in the C. elegans using a fluorescent and a confocal microscopes. From experimental results, the viabilities were achieved in 71 % (12/17) and 62% (8/13) at 10 μm and 20 μm different injection depths.