Abstract
In embryology, researchers mundanely identify specific protein on embryos using monoclonal antibodies, however the process of immunostaining is complex and takes long time by comparison with conventional methods. We have proposed a microfluidic device that can allow multi-embryos to expose to different reagents simultaneously. The device has 16 inlets and reaction chambers, and solutions are applied to them by use of a syringe pump. However, cost of using the syringe pump units and microfluidic device is expensive. The purpose of this study is to decrease running cost of the device for its commercialization. We tested usage of a low cost micro-ring pump and reuse of the device. As the result, we achieved to reduce more than 80% of the cost.
