Minimally Invasive Embryo Manipulating by Electrically Induced Bubbles

Abstract

For the present study, minimally-invasive embryo manipulation was carried out by using electrically induced bubbles with microfluidic chip. The procedure of somatic cell cloning consists of the removal of the nuclear from unfertilized egg and the injection of donor cells. Generally, these processes are carried out using a glass capillary called micro-injection, but this method tends to have a low reproducibility because skilled operator techniques are required. Also, high throughput embryo manipulations are required in livestock sector. Thus, this paper proposed a novel processing method of cloning with electrically-induced bubbles for minimally-invasiveness and high throughput process. By using this method, it is expected to perform injection of somatic cell in a short time and contribute to the high-throughput process of somatic cell cloning processes.