Capturing System of Well-focused Images of Moving Micro Objects Using Histograms of the Luminance

Abstract

In recent years, experiments using microscope and microchannel has been activity conducted. However it is difficult to focus the high-speed micro objects through microchannel because general height of microchannel is about 10-100 μm, but depth of focus of object lens is about 1-4 μm. Therefore, we propose the system attaching the vibration machine to object lens to move the focus position rapidly by vibrating it up and down, and capturing well-focused images using histogram-based algorithm. The result of experiments verify the efficacy of our capturing well-focused images of high-speed micro moving objects system.