Function Control of Optically-Driven Fluorescence Sensor with Multiple Wavelength Lights

Abstract

Control of function of a specific microsensor has a potential for single cell analysis with micro-nano robotic approach. We propose a new method of optical function control of the fluorescence microsensors such as manipulation, measurement, zeta potential control, and local heating using surface plasmon resonance with multiple wavelength lights. The microsensor is fabricated by modification of a fluorescence polystyrene microbead by gold nanorod and encapsulating the modified microbead into a lipid layer with 1,3,3-trimethylindolino-6'–nitrobenzopyrylospiran (SP), which is a photochromic material. This fluorescence microsensor is manipulated by optical tweezers with 1064 nm laser. Measurement of local environment is measured using fluorescence indicator in the microsensor. The zeta potential of the fluorescence microsensor switches from negative to positive by photo-isomerization of SP with ultraviolet irradiation. The fluorescence microsensor is heated by surface plasmon resonance with 808 nm laser. As demonstrations, each function of fluorescence microsensor was confirmed with multiple wavelength lights.